134 research outputs found

    Regulation of base excision repair: Ntg1 nuclear and mitochondrial dynamic localization in response to genotoxic stress

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    Numerous human pathologies result from unrepaired oxidative DNA damage. Base excision repair (BER) is responsible for the repair of oxidative DNA damage that occurs in both nuclei and mitochondria. Despite the importance of BER in maintaining genomic stability, knowledge concerning the regulation of this evolutionarily conserved repair pathway is almost nonexistent. The Saccharomyces cerevisiae BER protein, Ntg1, relocalizes to organelles containing elevated oxidative DNA damage, indicating a novel mechanism of regulation for BER. We propose that dynamic localization of BER proteins is modulated by constituents of stress response pathways. In an effort to mechanistically define these regulatory components, the elements necessary for nuclear and mitochondrial localization of Ntg1 were identified, including a bipartite classical nuclear localization signal, a mitochondrial matrix targeting sequence and the classical nuclear protein import machinery. Our results define a major regulatory system for BER which when compromised, confers a mutator phenotype and sensitizes cells to the cytotoxic effects of DNA damage

    Testing Above- and Below-Canopy Representations of Turbulent Fluxes in an Energy Balance Snowmelt Model

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    Turbulent fluxes of sensible and latent heat are important processes in the surface energy balance that drives snowmelt. Modeling these fluxes in a forested environment is complicated because of the canopy effects on the wind field. This paper presents and tests a turbulent flux model developed to represent these processes in an energy balance snowmelt model. The goal is to model these processes using the readily available inputs of canopy height and leaf area index in a way that minimizes the number of parameters, state variables, and assumptions about hard to quantify processes. Selected periods from 9 years of eddy-covariance (EC) measurements at Niwot Ridge, Colorado, were used to evaluate the effectiveness of this modeling approach. The model was able to reproduce the above-canopy sensible and latent heat fluxes reasonably with the correlation higher for sensible heat than latent heat. The modeled values of the below-canopy latent heat fluxes also matched the EC-measured values. The model captured the nighttime below-canopy sensible heat flux quite well, but there were discrepancies in daytime sensible heat flux possibly due to mountain slope circulation not quantifiable in this kind of model. Despite the uncertainties in the below-canopy sensible heat fluxes, the results are encouraging and suggest that reasonable predictions of turbulent flux energy exchanges and subsequent vapor losses from snow in forested environments can be obtained with a parsimonious single-layer representation of the canopy. The model contributes an improved physically based capability for predicting the snow accumulation and melt in a forested environment

    Author Correction: The FLUXNET2015 dataset and the ONEFlux processing pipeline for eddy covariance data

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    The FLUXNET2015 dataset and the ONEFlux processing pipeline for eddy covariance data

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    The FLUXNET2015 dataset provides ecosystem-scale data on CO2, water, and energy exchange between the biosphere and the atmosphere, and other meteorological and biological measurements, from 212 sites around the globe (over 1500 site-years, up to and including year 2014). These sites, independently managed and operated, voluntarily contributed their data to create global datasets. Data were quality controlled and processed using uniform methods, to improve consistency and intercomparability across sites. The dataset is already being used in a number of applications, including ecophysiology studies, remote sensing studies, and development of ecosystem and Earth system models. FLUXNET2015 includes derived-data products, such as gap-filled time series, ecosystem respiration and photosynthetic uptake estimates, estimation of uncertainties, and metadata about the measurements, presented for the first time in this paper. In addition, 206 of these sites are for the first time distributed under a Creative Commons (CC-BY 4.0) license. This paper details this enhanced dataset and the processing methods, now made available as open-source codes, making the dataset more accessible, transparent, and reproducible.Peer reviewe

    What Does Musicology Have to Do With Archiving? Three Experiences of Engagement

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    Musical practices derived from post-1960s experimental music created heterogeneous musical materials and traces—including scores, preparations and instrument modifications, electronic instruments, custom-made devices, and recordings. The Romantic work concept on which most traditional musical archives are based is unsuitable to preserve this expanded apparatus of objects and concepts, and rethinking the musical archive is becoming urgent.This colloquy collected the experiences of three researchers, engaging with five institutions, three creators, and four countries. Yet the archival issues presented are eerily similar. These experiences involve David Tudor (paper-based archive at the Getty Research Institute, Los Angeles, CA, and the David Tudor Instrument Collection at Wesleyan University, Midtown, CT); Mario Bertoncini (paper-based archive at the archive of the Akademie der KĂŒnste, Berlin, and his object collection at the moment stored at the Fondazione Isabella Scelsi, Rome); Gayle Young (who still owns all her production).Les pratiques musicales dĂ©rivĂ©es de la musique expĂ©rimentale depuis les annĂ©es 1960 ont produit des matĂ©riaux et des traces musicales hĂ©tĂ©rogĂšnes, comprenant des partitions, des prĂ©parations et modifications d’instruments, des dispositifs personnalisĂ©es, des enregistrements. Le concept d’oeuvre sur lequel la plupart des archives musicales traditionnelles se fondent, remontant au romantisme, n’est pas adaptĂ© pour prĂ©server ce complexe Ă©largi d’objets et de concepts, et il est de plus en plus urgent de repenser les archives musicales.Cette discussion rapproche les expĂ©riences de trois chercheur·e·s, qui s’intĂ©ressent Ă  cinq institutions et trois crĂ©ateur·rice·s dans quatre pays diffĂ©rents. Pourtant, les problĂšmes relatifs aux archives qu’on rencontre prĂ©sentent des ressemblances troublantes. Ces expĂ©riences regardent : David Tudor (archives papier au Getty Research Institute Ă  Los Angeles, Californie, et David Tudor Instrument Collection Ă  la Wesleyan University Ă  Midtown, Connecticut); Mario Bertoncini (archives papier aux archives de l’Akademie der KĂŒnste Ă  Berlin et collection d’objets hĂ©bergĂ©e actuellement Ă  la Fondazione Isabella Scelsi Ă  Rome); Gayle Young (qui est toujours en possession de toute sa production)
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